That is not an easy answer that can be dropped in this space. I have attached some code that does a five parameter logistic regression for a cell based bioassay that might be adaptable, but beware, it is not documented, it does get convoluted, and it was written for v9.1.3.
ANOVA, or Analysis Of Variance, is used to compare the averages or means of two or more populations to better understand how they differ. Watch this tutorial for more.