Hi, I am carrying out a research from an already established field experiment (Split plot design RCBD), Grass cultivars = whole plot, 3 levels, and legume cultivars are subplots with 5 levels. However, I only selected one grass cultivar and two legume cultivars for my current experiment resulting in Grass cultivar =whole plot, 1 level. Legume cultivars = subplot, 2 levels. Sample collection = 2 time points. Since I am using only one level of grass cultivar, is it okay to proceed with the data analysis using the split plot ANOVA model? If not, what is the best approach for my statistical analysis? Thanks.
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