Were the two houses at each farm assigned to some sort of treatment (for example, light cycle)? In other words, what do the two houses represent in your study? Does the level of house (1 or 2, 3 or 4, 5 or 6) affect the mean of the response or the variance of the response. Are you specifically interested in these 6 houses, or are they meant to represent "houses" in general?
Does the level of farm (1 or 2 or 3) affect the mean of the response or the variance of the response? Are you specifically interested in these 3 farms, or are they meant to represent "farms" in general?
I recommend this paper if you are unfamiliar with figuring out what factors are random and what factors are fixed Use and Misuse of Mixed Model Analysis of Variance in Ecological Studies It's biologically friendly 🙂
Were there groups of birds within each house, where each group of birds was assigned to a different method? If so, were the methods assigned at the same time, or in sequence? How many birds were in each group? Were different birds assigned to different groups?
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