Based on the sample data you provided, I've tried to sleuth out your design. For what it's worth, I attach my code.   I do not intend these comments to be critical (because we all start without knowing anything) but there appears to be many design concepts that you are unfamiliar with. If you have access to a statistical consultant at your institution, my strong recommendation is that you work with them to improve your understanding of experimental design and to develop an appropriate statistical model for your experiment.   If you have absolutely no access to statistical expertise, let us know and we'll see what we can do. But keep in mind that the answer to your question is truly and well beyond what we can readily address in this forum.   I hope this helps move you forward.   ... View more