There is no room for error in your model, or in other words, you used up all your DFs. The terms are Overall mean : 1 df treatment means : 3 df replicate means : 3 df interaction treatment x replicate means : 3x3 = 9 df Total : 16 df = nb of observations Your model has enough terms to fit each observation exactly. Without an estimate of error, you cannot derive p-values. PG
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