Dear Reeza, Thank you so much. However TRIM does not work. Please see below. DATA TST; INPUT Seq $; SeqT=TRIM(Seq); Notalphap=NOTALPHA(Seq); NotalphaTp=NOTALPHA(SeqT); Seql=LENGTH(Seq); SeqTl=LENGTH(SeqT); DATALINES; AbB1 aaaa Bcc Cdd ; RUN; PROC SQL; SELECT Seq, SeqT, Notalphap, NotalphaTp, Seql, SeqTl FROM Tst; QUIT; I got Seq SeqT Notalphap NotalphaTp Seql SeqTl AbB1 AbB1 4 4 4 4 AbB1a AbB1a 4 4 5 5 aaaa aaaa 5 5 4 4 Bcc Bcc 4 4 3 3 Cdd Cdd 4 4 3 3
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