About vitaaquaticus

vitaaquaticus · ‎02-13-2022

I am a new-ish SAS user and I tried following the instructions you linked. I ran the first three sections and I got several error messages such as "SELECT" statement is not valid. The instructions are a bit overwhelming for me and I am unclear on how to properly provide data. I am sorry for my ineptitude. Option E for Treatment Model: Option E for Light/Herbivory Model:

vitaaquaticus · ‎02-13-2022

I am not sure how to read the E option results, especially with the row numbers. I have included data in the original post if you want to sleuth a bit more and help me figure this out.

vitaaquaticus · ‎02-13-2022

I want to run a statistical model assessing the effects of light and herbivory on the length of plants (n = 53 observations). I developed one model with Site|Treatment as fixed effects (with Treatment containing both light and herbivory factors into one (-He = herbivores absent, +He = herbivores present, -Sh = unshaded, +Sh = shaded). Then, I tried a different model with Site|Light|Herbivory as fixed effects (splitting Treatment into two separate factors: light and herbivory). See the screenshot below. Even though the models are coded a bit differently, I would imagine that LSMESTIMATE should arrive at the same least squares mean values for both models considering they will calculate the mean of the response variable based on the same contrasts. However, I keep getting drastically different results for these two with respect to magnitude and direction of the means, and p-value? Treatment Model: DATA WORK.MONTH89; SET WORK.OR_LH_Under10_Index; IF Month >= 8 THEN OUTPUT; RUN; ods graphics on; PROC MIXED DATA = WORK.MONTH89 plots=all;; CLASS Treatment Site Month Block; MODEL'Change_Length'n = Site|Treatment /SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT; RANDOM Block Month /CL ALPHA=0.05 TYPE=VC; LSMESTIMATE Site*Treatment 'SH +He/-Sh x SH -He/-Sh'[1,2 2][-1,4 2], 'SH +He/+Sh x SH -He/+Sh'[1,1 2][-1,3 2], 'SH +He/-Sh x SH +He/+Sh'[1,2 2][-1,1 2], 'SH -He/-Sh x SH -He/+Sh'[1,4 2][-1,3 2], 'SH +He/-Sh x SH -He/+Sh'[1,2 2][-1,3 2], 'CB +He/-Sh x CB -He/-Sh'[1,2 1][-1,4 1], 'CB +He/+Sh x CB -He/+Sh'[1,1 1][-1,3 1], 'CB +He/-Sh x CB +He/+Sh'[1,2 1][-1,1 1], 'CB -He/-Sh x CB -He/+Sh'[1,4 1][-1,3 1], 'CB +He/-Sh x CB -He/+Sh'[1,2 1][-1,3 1] / CL Alpha=0.05; LSMESTIMATE Site 'SH x CB'[1,2][-1,1] / CL Alpha=0.05; RUN; ods graphics off; Light/Herbivory Model: DATA WORK.MONTH89; SET WORK.OR_LH_Under10_Index; IF Month >= 8 THEN OUTPUT; RUN; ods graphics on; PROC MIXED DATA = WORK.MONTH89 plots=all; CLASS Light Herbivory Month Block Site; MODEL 'Change_Length'n = Site|Light|Herbivory /SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT; RANDOM Block Month /CL ALPHA=0.05 TYPE=VC; LSMESTIMATE Site*Light*Herbivory 'Herbivory: SH Unshaded Present x Unshaded Absent'[1,2 2 2][-1,2 2 1], 'Herbivory: SH Shaded Present x Shaded Absent'[1,2 1 2][-1,2 1 1], 'Light: SH Unshaded Present x Shaded Present'[1,2 2 2][-1,2 1 2], 'Light: SH Unshaded Absent x Shaded Absent'[1,2 2 1][-1,2 1 1], 'H x L: SH Shaded Present x Unshaded Absent'[1,2 1 2][-1,2 2 1], 'Herbivory: CB Unshaded Present x Unshaded Absent'[1,1 2 2][-1,1 2 1], 'Herbivory: CB Shaded Present x Shaded Absent'[1,1 1 2][-1,1 1 1], 'Light: CB Unshaded Present x Shaded Present'[1,1 2 2][-1,1 1 2], 'Light: CB Unshaded Absent x Shaded Absent'[1,1 2 1][-1,1 1 1], 'H x L: CB Unshaded Present x Shaded Absent'[1,1 2 2][-1,1 1 1] / CL Alpha=0.05; RUN; ods graphics off; I am not sure if this is a content question or a programming question. I am hoping someone can help me understand what is happening behind the scenes.

vitaaquaticus · ‎04-20-2020

Ah yes, these pesky little commas! Thank you so much!!

vitaaquaticus · ‎04-19-2020

Okay, thank you so much for your help! I will reach out to the technical support.

vitaaquaticus · ‎04-19-2020

Cape has 3 levels (CF, CB and CP) and Year has 3 levels (2016, 2017, and 2018). The code runs fine without the LSMESTIMATE statement.

vitaaquaticus · ‎04-19-2020

Seems like the single quote is not the issue here. I tried to re-write the code from scratch and compact them together in fewer lines. Here's the error message: 1 The SAS System 07:33 Sunday, April 19, 2020 1 ;*';*";*/;quit;run; 2 OPTIONS PAGENO=MIN; 3 %LET _CLIENTTASKLABEL='SS_Transects'; 4 %LET _CLIENTPROCESSFLOWNAME='Process Flow'; 5 %LET _CLIENTPROJECTPATH='\\Mac\Home\Desktop\ElNinoKelpTransect.egp'; 6 %LET _CLIENTPROJECTPATHHOST='BARBARASPIEAEBF'; 7 %LET _CLIENTPROJECTNAME='ElNinoKelpTransect.egp'; 8 %LET _SASPROGRAMFILE=''; 9 %LET _SASPROGRAMFILEHOST=''; 10 11 ODS _ALL_ CLOSE; 12 OPTIONS DEV=PNG; 13 GOPTIONS XPIXELS=0 YPIXELS=0; 14 FILENAME EGSR TEMP; 15 ODS tagsets.sasreport13(ID=EGSR) FILE=EGSR 16 STYLE=HtmlBlue 17 STYLESHEET=(URL="file:///C:/Program%20Files%20(x86)/SASHome/x86/SASEnterpriseGuide/7.1/Styles/HtmlBlue.css") 18 NOGTITLE 19 NOGFOOTNOTE 20 GPATH=&sasworklocation 21 ENCODING=UTF8 22 options(rolap="on") 23 ; NOTE: Writing TAGSETS.SASREPORT13(EGSR) Body file: EGSR 24 25 GOPTIONS ACCESSIBLE; 26 ods graphics on; 27 PROC MIXED DATA = WORK.Data_Final_noAugust_SS plots(MAXPOINTS=none)=all method=REML; 28 CLASS Year Month Cape Site Transect Quadrat; NOTE: PROCEDURE MIXED used (Total process time): real time 0.00 seconds cpu time 0.00 seconds NOTE: The SAS System stopped processing this step because of errors. WARNING: The data set WORK.SMOOTH may be incomplete. When this step was stopped there were 0 observations and 0 variables. WARNING: Data set WORK.SMOOTH was not replaced because this step was stopped. 29 MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT outpred=Smooth; 30 RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; 31 LSMESTIMATE Year*Cape '2016 CB x 2017 CB'[1,1,2][-1,1,1], _ _ 22 22 200 200 32 '2017 CB x 2018 CB'[1,2,3][-1,1,1]/ ADJUST=BON; _ _ 22 22 200 200 ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored. 33 RUN; 34 ods graphics off; 35 36 GOPTIONS NOACCESSIBLE; 37 %LET _CLIENTTASKLABEL=; 38 %LET _CLIENTPROCESSFLOWNAME=; 39 %LET _CLIENTPROJECTPATH=; 40 %LET _CLIENTPROJECTPATHHOST=; 2 The SAS System 07:33 Sunday, April 19, 2020 41 %LET _CLIENTPROJECTNAME=; 42 %LET _SASPROGRAMFILE=; 43 %LET _SASPROGRAMFILEHOST=; 44 45 ;*';*";*/;quit;run; 46 ODS _ALL_ CLOSE; 47 48 49 QUIT; RUN; 50

vitaaquaticus · ‎04-19-2020

It adds another error when I put Year*Cape after the text with quotation marks. 1 The SAS System 07:33 Sunday, April 19, 2020 1 ;*';*";*/;quit;run; 2 OPTIONS PAGENO=MIN; 3 %LET _CLIENTTASKLABEL='SS_Transects'; 4 %LET _CLIENTPROCESSFLOWNAME='Process Flow'; 5 %LET _CLIENTPROJECTPATH='\\Mac\Home\Desktop\ElNinoKelpTransect.egp'; 6 %LET _CLIENTPROJECTPATHHOST='BARBARASPIEAEBF'; 7 %LET _CLIENTPROJECTNAME='ElNinoKelpTransect.egp'; 8 %LET _SASPROGRAMFILE=''; 9 %LET _SASPROGRAMFILEHOST=''; 10 11 ODS _ALL_ CLOSE; 12 OPTIONS DEV=PNG; 13 GOPTIONS XPIXELS=0 YPIXELS=0; 14 FILENAME EGSR TEMP; 15 ODS tagsets.sasreport13(ID=EGSR) FILE=EGSR 16 STYLE=HtmlBlue 17 STYLESHEET=(URL="file:///C:/Program%20Files%20(x86)/SASHome/x86/SASEnterpriseGuide/7.1/Styles/HtmlBlue.css") 18 NOGTITLE 19 NOGFOOTNOTE 20 GPATH=&sasworklocation 21 ENCODING=UTF8 22 options(rolap="on") 23 ; NOTE: Writing TAGSETS.SASREPORT13(EGSR) Body file: EGSR 24 25 GOPTIONS ACCESSIBLE; 26 ods graphics on; 27 PROC MIXED DATA = WORK.Data_Final_noAugust_SS plots(MAXPOINTS=none)=all method=REML; 28 CLASS Year Month Cape Site Transect Quadrat; 29 MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT outpred=Smooth; 30 RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; 31 LSMESTIMATE "2016 CB x 2017 CB" Year*Cape ___________________ 22 200 NOTE: PROCEDURE MIXED used (Total process time): real time 0.01 seconds cpu time 0.00 seconds NOTE: The SAS System stopped processing this step because of errors. WARNING: The data set WORK.SMOOTH may be incomplete. When this step was stopped there were 0 observations and 0 variables. WARNING: Data set WORK.SMOOTH was not replaced because this step was stopped. ERROR 22-322: Expecting a name. ERROR 200-322: The symbol is not recognized and will be ignored. 32 [1,1,2] _ 22 200 33 [-1,1,1], _ 22 200 ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored. 34 "2017 CB x 2018 CB" 35 [1,2,3] 2 The SAS System 07:33 Sunday, April 19, 2020 _ 22 200 36 [-1,1,1]/ ADJUST=BON; _ 22 200 ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored. 37 RUN; 38 ods graphics off; 39 40 GOPTIONS NOACCESSIBLE; 41 %LET _CLIENTTASKLABEL=; 42 %LET _CLIENTPROCESSFLOWNAME=; 43 %LET _CLIENTPROJECTPATH=; 44 %LET _CLIENTPROJECTPATHHOST=; 45 %LET _CLIENTPROJECTNAME=; 46 %LET _SASPROGRAMFILE=; 47 %LET _SASPROGRAMFILEHOST=; 48 49 ;*';*";*/;quit;run; 50 ODS _ALL_ CLOSE; 51 52 53 QUIT; RUN; 54

vitaaquaticus · ‎04-19-2020

Here's the log: 1 The SAS System 07:33 Sunday, April 19, 2020 1 ;*';*";*/;quit;run; 2 OPTIONS PAGENO=MIN; 3 %LET _CLIENTTASKLABEL='SS_Transects'; 4 %LET _CLIENTPROCESSFLOWNAME='Process Flow'; 5 %LET _CLIENTPROJECTPATH='\\Mac\Home\Desktop\ElNinoKelpTransect.egp'; 6 %LET _CLIENTPROJECTPATHHOST='BARBARASPIEAEBF'; 7 %LET _CLIENTPROJECTNAME='ElNinoKelpTransect.egp'; 8 %LET _SASPROGRAMFILE=''; 9 %LET _SASPROGRAMFILEHOST=''; 10 11 ODS _ALL_ CLOSE; 12 OPTIONS DEV=PNG; 13 GOPTIONS XPIXELS=0 YPIXELS=0; 14 FILENAME EGSR TEMP; 15 ODS tagsets.sasreport13(ID=EGSR) FILE=EGSR 16 STYLE=HtmlBlue 17 STYLESHEET=(URL="file:///C:/Program%20Files%20(x86)/SASHome/x86/SASEnterpriseGuide/7.1/Styles/HtmlBlue.css") 18 NOGTITLE 19 NOGFOOTNOTE 20 GPATH=&sasworklocation 21 ENCODING=UTF8 22 options(rolap="on") 23 ; NOTE: Writing TAGSETS.SASREPORT13(EGSR) Body file: EGSR 24 25 GOPTIONS ACCESSIBLE; 26 ods graphics on; 27 PROC MIXED DATA = WORK.Data_Final_noAugust_SS plots(MAXPOINTS=none)=all method=REML; 28 CLASS Year Month Cape Site Transect Quadrat; 29 MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT outpred=Smooth; 30 RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; 31 LSMESTIMATE Year*Cape "2016 CB x 2017 CB" NOTE: PROCEDURE MIXED used (Total process time): real time 0.00 seconds cpu time 0.00 seconds NOTE: The SAS System stopped processing this step because of errors. WARNING: The data set WORK.SMOOTH may be incomplete. When this step was stopped there were 0 observations and 0 variables. WARNING: Data set WORK.SMOOTH was not replaced because this step was stopped. 32 [1,1,2] _ 22 200 33 [-1,1,1], _ 22 200 ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored. 34 "2017 CB x 2018 CB" 35 [1,2,3] _ 22 200 36 [-1,1,1]/ ADJUST=BON; _ 2 The SAS System 07:33 Sunday, April 19, 2020 22 200 ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored. 37 RUN; 38 ods graphics off; 39 40 GOPTIONS NOACCESSIBLE; 41 %LET _CLIENTTASKLABEL=; 42 %LET _CLIENTPROCESSFLOWNAME=; 43 %LET _CLIENTPROJECTPATH=; 44 %LET _CLIENTPROJECTPATHHOST=; 45 %LET _CLIENTPROJECTNAME=; 46 %LET _SASPROGRAMFILE=; 47 %LET _SASPROGRAMFILEHOST=; 48 49 ;*';*";*/;quit;run; 50 ODS _ALL_ CLOSE; 51 52 53 QUIT; RUN; 54

vitaaquaticus · ‎04-19-2020

Thank you for the documentation. I read it and I am having difficulty understanding how to write the code exactly. For example: ods graphics on; PROC MIXED DATA = WORK.Data_Final_noAugust_SS plots(MAXPOINTS=none)=all method=REML; CLASS Year Month Cape Site Transect Quadrat; MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT outpred=Smooth; RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; LSMESTIMATE Year*Cape "2016 CB x 2017 CB" [1,1,2] [-1,1,1], "2017 CB x 2018 CB" [1,2,3] [-1,1,1]/ ADJUST=BON; RUN; ods graphics off; I keep getting error messages. For this particular code, I get this for the brackets: ERROR 22-322: Syntax error, expecting one of the following: a numeric constant, a datetime constant, +, -, ]. ERROR 200-322: The symbol is not recognized and will be ignored.

vitaaquaticus · ‎04-19-2020

I am trying to specify several pre-planned comparisons for my PROC MIXED model and using a Bonneferoni adjustment for these comparisons instead of comparing every possible combination using Tukey. The SAS literature says: "You can specify multiple effects in one LSMEANS statement or in multiple LSMEANS statements, and all LSMEANS statements must appear after the MODEL statement" How do I specifically list the individual comparisons under one LSMEANS statement and have them be adjusted together as one unit? For example: ods graphics on; PROC MIXED DATA = WORK.Data_Final_noAugust_SS plots(MAXPOINTS=none)=all method=REML; CLASS Year Month Cape Site Transect Quadrat; MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT outpred=Smooth; RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; LSMEANS Year|Cape / PDIFF CL ALPHA=0.05 ADJUST=TUKEY; RUN; ods graphics off; Year*Cape compares every single combination of Cape (three levels: CF, CP, CB) and Year (three levels: 2016, 2017, 2018). I do not want that; instead, I want specific comparisons with the Bonneferoni adjustment: For example: CB 2016 vs CB 2017 CB 2017 vs CB 2018 CF 2016 vs CB 2016 CP 2016 vs CB 2016 2016 vs 2017 2017 vs 2018 CF vs CB CF vs CP CB vs CP How do I write that? Thank you!

vitaaquaticus · ‎04-01-2020

I ran two exactly same models using PROC GLIMMIX and PROC MIXED. I specify both PROCs to use a Gaussian distribution. I looked up the literature and they said that these two models with the same Gaussian distribution should yield similar results? However, I got two different results with respect to residuals (using plots as visual references). Why is this the case? Thank you!! PROC GLIMMIX model ods graphics on; PROC GLIMMIX DATA = WORK.Data_Final_noAugust_EM plots=all METHOD=RSPL; CLASS Year Month Cape Site Transect Quadrat; MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT DIST=GAUSSIAN; RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; LSMEANS Year|Cape / PDIFF CL ALPHA=0.05; RUN; ods graphics off; PROC MIXED model ods graphics on; PROC MIXED DATA = WORK.Data_Final_noAugust_EM plots(MAXPOINTS=none)=all method=REML; CLASS Year Month Cape Site Transect Quadrat; MODEL 'Percent.Cover'n = Month Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT; RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; LSMEANS Year|Cape / PDIFF CL ALPHA=0.05; RUN; ods graphics off;

vitaaquaticus · ‎02-19-2020

Cape is a regional level. Site is a local level. It is like a city within a county. But in this case, a site within a cape.

vitaaquaticus · ‎02-19-2020

Thanks for the suggestion. I just tried it - it shows a very similar residual vs predicted plot.

vitaaquaticus · ‎02-19-2020

I have a dataset that contains three response variables (density of individuals in a 0.5 m^2 plot, length of the longest individual in the 0.5 m^2 plot, and percent cover of a 0.5 m^2 plot). The sample sizes are very small so I fitted a PROC MIXED statistical model for each response variable with REML and Kenward-Roger correction. I am having troubles finding a way to satisfy the model assumptions with respect to heteroscedasticity and normality. The residual vs predicted mean plot shows a diagonal shape (see photos below) with all of the transformations (log, sqrt, power, etc). I thought if I fit a beta distribution for percent cover and a poisson distribution for density, it might help solve the problem. But the issue is - PROC MIXED does not have the capability to specify a distribution. PROC GLIMMIX does but it does not appear to have the capability to specify REML and Kenward-Roger correction. I am a bit stumped at this point. I am hoping someone can help me on this. Thank you! Sample Dataset: data WORK.DATASET2; infile datalines dsd truncover; input F1:BEST4. Year:$4. Month:$5. Date:MMDDYY10. Cape:$2. Site:$2. Species:$19. Transect:$1. Quadrat:$2. Percent.Cover:BEST12. Density:BEST12. Frond.Length:BEST5. Length_75:32.; format F1 BEST4. Date MMDDYY10. Percent.Cover BEST12. Density BEST12. Frond.Length BEST5.; datalines; 1 2016 May 05/06/2016 CF BB Saccharina sessilis 1 1 30 9 16.5 8.1867769506 2 2016 May 05/06/2016 CF BB Saccharina sessilis 1 2 51 11 26 11.514100371 3 2016 May 05/06/2016 CF BB Saccharina sessilis 1 3 14 5 13 6.846325042 4 2016 May 05/06/2016 CF BB Saccharina sessilis 1 4 3 1 8 4.75682846 5 2016 May 05/06/2016 CF BB Saccharina sessilis 1 5 4 1 2.1 1.7444738796 6 2016 May 05/06/2016 CF BB Saccharina sessilis 1 6 17 4 25.4 11.314237411 7 2016 May 05/06/2016 CF BB Saccharina sessilis 1 7 15 8 14 7.2376241554 8 2016 May 05/06/2016 CF BB Saccharina sessilis 1 8 21 4 23 10.502577066 9 2016 May 05/06/2016 CF BB Saccharina sessilis 1 9 16 5 9.5 5.4111890111 10 2016 May 05/06/2016 CF BB Saccharina sessilis 1 10 24 6 8.9 5.1527907317 11 2016 May 05/06/2016 CF BB Saccharina sessilis 1 11 17 5 2 1.6817928305 12 2016 May 05/06/2016 CF BB Saccharina sessilis 1 12 52 9 18.1 8.7752385459 13 2016 May 05/06/2016 CF BB Saccharina sessilis 1 13 17 2 13.8 7.1599388764 14 2016 May 05/06/2016 CF BB Saccharina sessilis 1 14 30 7 26 11.514100371 15 2016 May 05/06/2016 CF BB Saccharina sessilis 1 15 62 14 33.7 13.986930782 16 2016 May 05/06/2016 CF BB Saccharina sessilis 1 16 30 9 26 11.514100371 17 2016 May 05/06/2016 CF BB Saccharina sessilis 1 17 20 10 16.1 8.0374707792 18 2016 May 05/06/2016 CF BB Saccharina sessilis 1 18 6 3 8 4.75682846 19 2016 May 05/06/2016 CF BB Saccharina sessilis 1 19 10 3 11.1 6.0812412691 20 2016 May 05/06/2016 CF BB Saccharina sessilis 1 20 18 8 2.5 1.9881768219 ;;;; Sample code for Density: ods graphics on; PROC MIXED DATA = dataset2 plots(MAXPOINTS=none)=all; CLASS Year Month Cape Site Transect Quadrat; MODEL 'Density'n = Year Month(Year) Year|Cape/SOLUTION ddfm = KR CL ALPHA=0.05 INTERCEPT; RANDOM Quadrat(Transect) Transect(Site) Site(Cape) /CL ALPHA=0.05 TYPE=VC; LSMEANS Year|Cape / PDIFF CL ALPHA=0.05; RUN; ods graphics off;

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