@PGStats What is your opinion on the importance of normality for the LS-means (in a mixed procedure)? Does the departure from normality make much effect in LS-means? ... View more

@Rick_SASand @PGStats, I really appreciate further support. ... View more

  Hi Rick,   This would be my code for the ANOVA model ( 5 samples, 177 Entries and 2 blocks). proc mixed data=one covtest cl;                                                               class Sample Entry  Block;                                                        model Measure = Sample  Entry  Sample*Entry/ddfm=kenwardroger;                                   random Block;                                                   run;        Please ignore this sentence  'In my experiment, dependent or independent variables are not defined'.Measure is the dependent variable in the model statement. Before performing ANOVA, I  checked the normality for the residuals in individual samples. Please check some of the results of the residual analysis in my reply to PG. Any advice is much appreciated. Thanks   ... View more

Hello PG,   Thanks for your reply. I have 5 samples(datasets), each sample size is 177 with 2 blocks. The model for the ANOVA is the mixed model considering the blocks as the random factor. Here I have attached two histograms and plots of residual*predicted for two datasets. The Kurtosis values were way too high ( ex: 8.1). Shapiro-Wilk test was considered as the test for normality and the Pr<W were mostly <0.0001. The residual*predicted plots appears in fan shaped.Do I still have the chance to use these sample data as it is without a transformation?   Thanks, Nadishika       ... View more