Hello, I am a beginner at SAS, I have done an experiment where I wanted to discover the amount of repeated observations per experimental unity is needed to correctly access the trait: distribution of bean root. The design chosen was randomized complete block.   The factors are: block (2); generations (F2 and F5) and number of plants evaluated per experimental unity (2 4 6 or 8). The plants were evaluated by opening trenches near plants and observing the presence or absence of roots at 60 5x5 cm squares per plant. It was given 1 for the square that presented at least 1 root and 0 if no root was visible.    So I want to know what is the best number of plants that I need to evaluate per experimental unit at each generations to have a reliable answer, what tests should I perform? I am using SAS 9.4.    Cleiton Wille Best regards ... View more