Thank you very much, I will try now to apply to my data. ... View more

I apologize for not clarifying things properly. At 0h I assume that the values obtained in one piece of tissue will be equal for all the groups (as the treatment hasn´t started yet) and I use those values at 0h for the rest of the groups from the same animal. So I have 12 pieces of tissue plus one for the 0h (please see image below that only represents animal 1).  All pieces are run at the same time. In the model, I am using (I use a .prn file were I take the values from) :                            alive/ Totals [the same as saying alive/ (dead+alive) ]. When I run the model I have negative values for the estimates (please see image below) that I´m having trouble interpreting but that  I guess they are the logit (have to convert in probabilities ?) ? Can you help me determining an appropriate model structure ? Thank you very much.   ... View more

And the same for the other cell types:   proc glimmix; class Vaca Grupo Horas; model PMVivos/PMTotais = Horas/dist =bin link =logit; random Vaca; lsmeans horas / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model PMVivos/PMTotais = Grupo/dist =bin link =logit; random Vaca; lsmeans Grupo / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model PVivos/Ptotais = Horas/dist =bin link =logit; random Vaca; lsmeans horas / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model PVivos/Ptotais = Grupo/dist =bin link =logit; random Vaca; lsmeans Grupo / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model SVivos/Stotais = Horas/dist =bin link =logit; random Vaca; lsmeans horas / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model SVivos/Stotais = Grupo/dist =bin link =logit; random Vaca; lsmeans Grupo / ilink pdiff; run; ... View more

Yes, here it goes what I have at the moment. Thanks proc glimmix; class Vaca Grupo Horas; model PMVivos/PMTotais = Horas/dist =bin link =logit; random Vaca; lsmeans horas / ilink pdiff; run; proc glimmix; class Vaca Grupo Horas; model PMVivos/PMTotais = Grupo/dist =bin link =logit; random Vaca; lsmeans Grupo / ilink pdiff; run; ##- Please type your reply above this line. Simple formatting, no attachments. -## ... View more

When I do the analysis to see if the cells are alive or dead, II have to "kill" that part of the in vitro cultured tissue sample. So I have to use different pieces of tissue that are not entirely equal between them (although coming from the same animal). So for example, I have a major piece of tissue from the same animal that I divide in the number of smaller pieces to use in the different groups of treatment and hours of treatment. Thank you ... View more

Sorry, I forgot to mention that Vivos means alive and Mortos means Dead (PM, P, and S are the 3 cell types), so, for exemple: Thank you Vaca Grupo Horas  PMVivos   PMMortos   1 1 0 9 9 PM cells alive in animal 1, group 1, with 0h of treatment 33 33 PM dead cells  in animal 1, group 1, with 0h of treatment 1 1 4 8 8 PM cells alive in animal 1, group 1, with 4h of treatment 9 9 PM dead cells in animal 1, group 1, with 4h of treatment 1 1 24 7 7 PM cells alive in animal 1, group 1, with 24h of treatment 44 44 PM dead cells in animal 1, group 1, with 24h of treatment ... View more

Sorry, no with the correct file attatched ... View more