Hi, I'm not confident in how to correctly go about specifying my modelin Proc Glimmix. This experiment was a RCBD with subsampling over time(3,27 hour time points). Below are my fixed and random effects. with/without peptides Class of peptide Peptide concentrations (5 or 1 levels) time (2 levels) block (4 levels) with CPP A 6.25,12.5,25,50,100 3,27 1,2,3,4 with CPP B 6.25,12.5,25,50,100 3,27 1,2,3,4 with CPP C 6.25,12.5,25,50,100 3,27 1,2,3,4 with CPP D 6.25,12.5,25,50,100 3,27 1,2,3,4 with CPP E 6.25,12.5,25,50,100 3,27 1,2,3,4 with CPP F 6.25,12.5,25,50,100 3,27 1,2,3,4 with non-CPP Control peptide 6.25,12.5,25,50,100 3,27 1,2,3,4 without - - 0 3,27 1,2,3,4 The treatment without peptides is essentially a control to know the value of my response variables at a concentration of 0 - it applies to every peptide since the concentration of peptides is 0. Should I repeatedly enter this data for each peptide, since the values are applicable to every peptide? Or is there a way to enter the data once per experimental unit that was measured such that it is applied as concentration level of 0 for each peptide? Does it make sense for 'with/without peptides' to be a variable? Another control in this experient is the control peptide. This peptide is of a different class than my other peptides. Correspondingly, I expect it to cause significant interactions as there is a different response with the control peptide. How do I include my data for the control peptide without compromising my ability to also analyse the significance of main effects and interactions within only the CPP class of peptides? Are any of my effects nested here? For example, 'class of peptides' nested in 'with/without peptides', or 'peptide' nested in 'class of peptides', and 'concentrations' nested in 'with/without' peptides? Any help would be appreciated. Thanks
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