Yes, you can if you think location is a fixed effect. If location is a random effect, I would think it is a split-plot design at multiple random locations, then you cannot test the p-value of location, and the random statement should not include Loc*subplot, Loc*mainplot*subplot, because that will give the wrong error term of F test for subplot.
ANOVA, or Analysis Of Variance, is used to compare the averages or means of two or more populations to better understand how they differ. Watch this tutorial for more.