The data I attached is from an experiment where I am measuring the stress response of 3 types of fish (trt: 1, 2, 3) over a 1440 minute (24 hour) time period. Fish were divided into 9 tanks (12 fish per tank, 3 tanks per fish type) and were chased for 1 minute using dip nets to elicit a stress response. At each time point since the chasing (time: 0, 20, 60, 120, 360, 1440 minutes) blood was sampled from 2 fish in each tank. I measured many response variables, but "osmo" (osmolality) is the one I provided in my post. I hypothesize: a) trt: 2 fish will exhibit significantly lower levels of osmo than trt: 1 and trt: 3 fish b) trt: 1 fish will not differ from trt: 3 fish c) there will be no interaction between trt and time
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