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Spatial prediction applied to lymphoid follicles

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Contributor das
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Posts: 44

Spatial prediction applied to lymphoid follicles

Sampling strategy.gif

I'm looking for advice on approach and application to spatial prediction. I'm a life scientist trying to spatially predict morphologic measures of lymphoid follicles (such as frequency and area) in a specialized region of the intestinal track. The spatial reference is distance coordinates (x,y) from an anatomical landmark. The sampling strategy is shown above where the black tube on the left represents the intestinal segment which is opened along the dotted grey line to be layed out flat as shown to the right as a larger grey retangular shape. The red line is the anantomical landmark from which the distance along the y-axis can be measured continuously, the blue rectangles extending vertical from the landmark represent the tissue sample strip that is microscopically assessed for follicles. Since this was originally a tube, my first thought is to consider the x-axis values as discrete clock face positions. The goal is to use this sampling strategy to spatially predict at any x,y coordinate the frequency and area of follicules.

My plan has been to use kriging procedures available in SAS. While the SAS help documentation is a good start, I wonder if someone could point me to a reference that might go into more detail, especially when it comes to assumption checking and application in what may be an unusual application of this procedure to tissue morphometrics. I love learning from examples that might replicate the essential qualities of my sampling approach. Also welcome any guidence to approaches other than kriging. For example, can anyone help me compare kriging with stereologic approaches, is my sampling and statistical plan appropriate and/or analogous.

Thank you,

Dave

SAS Super FREQ
Posts: 3,307

Re: Spatial prediction applied to lymphoid follicles

I found this SAS Global Forum paper to be readable:
Everything in Its Place: Efficient Geostatistical Analysis with SAS/STAT® Spatial Procedures

If you are up for a book-level treatment that is more theoretical and less code-oriented, I enjoyed reading Statistical Methods for Spatial Data Analysis by Schabenberger and Gotway. I think it is extremely well written.

Contributor das
Contributor
Posts: 44

Re: Spatial prediction applied to lymphoid follicles

Both look like excellent suggestions and what I need to start the ball rolling. I forgot to mention that I intend also to include a temporal factor (age of subject) as a separate research question. I see that the Amazon link you provided includes a suggested book "Statistics for Spatio-Temporal Data" and other similar titles are easily discovered. Looks like I've got my work cut out for me but its exciting to delve into a new area to solve these research questions. Thank you for your quick and helpful reply.

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